INDEX

A

Acetylated low density lipoprotein (AcLDL) uptake

assay..............................................................216

Achilles tendon..............................................................138

Aerobic microorganisms ................................................. 17

Allogenic therapies.......................................................... 87

Alvetex^® membranes...........................................243, 244,

251–253, 256

Anchorage-dependent cells ..........................................155

Animal derived component free (ADCF)........................ 2

Antibiotics .................................. 170–173, 176, 179–181

Arginine-glycine-aspartate (RGD) sequence................. 85

Autologous/allogeneic therapeutic approaches............ 84

B

Bacterial nanocellulose-based grafts, for cell colonization

studies ................................................. 205–207

materials

antibodies .......................................................... 208

assays and staining solutions............................. 208

bioreactor setup.........................................207–208

cell culture ......................................................... 208

chemicals and solutions .................................... 208

consumables ...................................................... 209

hardware ....................................................209–210

methods

AcLDL uptake assay.......................................... 216

acridine orange staining.................................... 218

bioreactor setup.........................................210–211

CD31 immunofluorescence staining .......218–219

cell seeding ................................................211–212

considerations.................................................... 210

lactate monitoring.....................................212–213

Phalloidin—FActin staining .....................216–218

small diameter vascular grafts, connection ...... 211

termination of experiment................................ 216

WST-1 proliferation assay.........................213–215

Bambanker™.................................................................107

BioBLU^® 0.3c bioreactor............................................... 86

BioBLU^® 5c stirred bioreactor ...................................... 91

Biological scaffolds........................................................180

Biomanufacturing of human stem cells .............. 193, 194

Bioreactor ............................................................. 127, 243

cell cultivation ............................................................. 2

commercially available single-use bioreactors ......... 84

gas transfer................................................................. 13

macrosparger ............................................................... 3

pitched blade impellers ............................................... 3

preparation .................................................................. 7

setpoints................................................................... 7–8

single-use bioreactors................................................ 84

stirred tank (see Stirred tank reactors (STR))

Bioreactor-based adherent cells harvesting ........ 257–259

3D printing technology-based method .................258

inertial microfluidics ...............................................258

materials

cell harvesting.................................................... 260

3D printing................................................259–260

PDMS device..................................................... 260

membrane-based technologies ...............................257

methods

device design .............................................260–261

direct fabrication ............................................... 261

mold fabrication ........................................261–262

operation....................................................262–263

PDMS ......................................................................265

PMMA sheet ...........................................................263

SLA printer..............................................................264

Biosafety cabinet (BSC)................................................168

C

Caco-2 cell line..............................................................245

Carbon cataboite repression (CCR) ............................224

Cardiomyocytes............................................................... 78

Cascaded continuous cultivation......................... 223–225

materials

host cells ............................................................ 225

required devices or cultivation .................225–227

required equipment for process

analysis .................................................227–228

required media .................................................. 225

methods

biomass determination..............................233–234

continuous adaptation phase....................230–232

cultivation setup ........................................228–229

determination of metabolite accumulation ..... 234

flow rates calculation......................................... 235

induction phase ................................................. 232

Kursad Turksen (ed.), Bioreactors in Stem Cell Biology: Methods and Protocols,

Methods in Molecular Biology, vol. 2436, https://doi.org/10.1007/978-1-0716-2018-2,

© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

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